Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: Scutellarin Inhibits Proliferation, Invasion, and Tumorigenicity in Human Breast Cancer Cells by Regulating HIPPO-YAP Signaling Pathway
doi: 10.12659/MSM.904492
Figure Lengend Snippet: Scutellarin impaired the invasion ability of human breast cancer MCF-7 cells. MCF-7 cells were cultured with moderate Scutellarin concentrations (80 μM) of Scutellarin in the upper Transwell chambers. Following 16 h of incubation, Matrigel was coated on the Transwell chambers. After 24 h, the invaded cells were photographed (×200) and quantified by a microplate reader. All data are represented as mean ±SD of 3 independent experiments. ** P<0.01, Scutellarin-treated group compared with the untreated control group.
Article Snippet: Cell invasion were determined using Transwell cell Matrigel invasion chambers (Matrigel-coated membrane, BD Biosciences, San Jose, CA, USA) as previously described [ ].
Techniques: Cell Culture, Incubation